Internal validation has been performed, demonstrating that our antibodies work well on a variety of applications: Western blot, ELISA, immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry (IHC), immunocytochemistry (ICC), flow cytometry (FC) and chromatin immunoprecipitation (CHIP). As protocols vary, some optimization may be necessary, part of a validation process can be qualifying more than one specifically targeted antibody in order to achieve the best possible result. We are eager to work with you to ensure that your protocols achieve the highest possible signal to noise ratios. Please let us know how we can help.
Western Blots: Our antibodies have been validated to provide the strongest signal with the least background, using a variety of standard western blotting protocols. As protocols do vary, some optimization may be required, but it should be minimal. Validating more than one antibody directed towards a single antigenic site may be necessary to achieve the best results.